Principles of Proteomics isВ designed specifically to explain the different stages of proteomic analysis, their complexities and their jargon to students and researchers in a non-technical overview of the field.
The author describes the broad range of problems which proteomics can address, including structural proteomics, interaction proteomics, protein modification analysis and functional proteomics.В Methodologies are described in user-friendly language, from the more traditional two-dimensional gel electrophoresis to the new developments in protein chip technologies.В These are well presented in the context of overall strategies which can be adopted to address the different aspects of large-scale protein analysis.
The
precise amino acid content, and the sequence of those amino acids, of
a specific protein, is determined by the sequence of the bases in the
gene that encodes that protein. The chemical properties of the amino acids
of proteins determine the biological activity of the protein. Proteins
not only catalyze all (or most) of the reactions in living cells, they
control virtually all cellular process. In addition, proteins contain
within their amino acid sequences the necessary information to determine
how that protein will fold into a three dimensional structure, and the
stability of the resulting structure. The field of protein folding and
stability has been a critically important area of research for years,
and remains today one of the great unsolved mysteries. It is, however,
being actively investigated, and progress is being made every day.
